Neutralization of Staphylococcal enterotoxin B by aptamer
نویسندگان
چکیده
12 Staphylococcal enterotoxin B (SEB) is a major virulence factor for 13 Staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T 14 lymphocytic population, releasing pro-inflammatory cytokines. Blocking 15 SEB-initiated toxicity may be an effective strategy for treating TSS. Using a 16 systematic evolution of ligands by exponential enrichment (SELEX) process, we 17 identified an aptamer that can antagonize SEB with nanomolar binding affinity (Kd = 18 64 nM). The aptamer antagonist effectively inhibits SEB-mediated proliferation and 19 cytokine secretion in human peripheral blood mononuclear cells. Moreover, a 20 PEGlyated aptamer antagonist significantly reduced mortality in a “double-hit” mouse 21 model of SEB-induced TSS, established via sensitization with D-galactosamine 22 followed by SEB challenge. Therefore, our novel aptamer antagonist may offer 23 potential therapeutic efficacy against SEB-mediated TSS. 24
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